Protein separation and purification using nanodiamonds is rapid, simple and efficient.
By using nanodiamonds recombinant apoobelin and recombinant luciferase are separated from bacterial cells of Escherichia coli.
Advantages of using nanodiamonds for protein separation
- Nanodiamonds simplify the protein purification procedures
- Nanodiamonds decrease protein separation time down to 30–40 min
- Nanodiamonds liminate the necessity of using special chromatographic equipment
- Nanodiamonds enable the preparing high-purity apoobelin and luciferase materials with protein yields of 35–45 and 45–60% respectively